Trio Breeding

Alternative breeding strategy in which two adult females and one adult male are co-housed. Unless otherwise approved, one of the adult females must be removed prior to parturition such that no more than two adults and one litter are in the cage at any given time. Justification for overcrowding may be submitted to the IACUC and if approved, trio birthing may be used. Singly housed pregnant females or stud males should be labeled with a blue tab as described in the Identifying Single Housed Animals SOP.