The guidance below provides information on how to appropriately sterilize examination gloves.
Supplies Needed
- Clean latex or nitrile exam gloves
- Paper drape material
- Sterilization pouches
- Autoclave indicator
- Autoclave
Step-by-Step Instructions
1. |
Assure that autoclave is in good working order, with periodic testing, assessment, and appropriate record-keeping (review the Monitoring Effectiveness of Autoclaves document for more information) |
2. |
Fold the wrists of each glove up over the hand of the glove |
3. |
Cut drape material into rectangles to fold around each pair of gloves |
4. |
Wrap gloves, one pair per packet, with drape material to separate pairs of gloves or to separate gloves from instruments |
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5. |
Place an autoclave indicator within each glove |
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6. |
Place up to 5 pairs of gloves in each sterilization pouch, or one pair with the instruments to be used for the surgery (Note: contrary to the picture, place gloves ON TOP OF instruments to assist in maintaining sterility) |
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7.
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Autoclave gloves
- Vacuum cycles
- Sterilize at 121°C for 20 minutes and dry 12 minutes
- Sterilize at 121°C for 30 minutes and dry 12 minutes
- Sterilize at 132°C for 6 minutes and dry 20 minutes
- Sterilize at 132°C for 3 minutes and dry 1 minute (flash cycle used only for gloves in a peel pouch -- no instruments)
- Gravity Cycle
- Sterilize 30 minutes and dry 10 minutes
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For more information about the preparation of surgical supplies for rodent surgery, including the use of surgical drapes and instruments, please refer to the Guidelines on the Performance of Surgery in Rodents.