{"id":3053,"date":"2018-08-23T13:20:06","date_gmt":"2018-08-23T13:20:06","guid":{"rendered":"https:\/\/az.research.umich.edu\/animalcare\/document\/helpful-information-sheet-sterile-gloves-rodent-surgery\/"},"modified":"2025-02-17T15:44:31","modified_gmt":"2025-02-17T15:44:31","slug":"helpful-information-sheet-sterile-gloves-rodent-surgery","status":"publish","type":"document","link":"https:\/\/az.research.umich.edu\/animalcare\/informational\/helpful-information-sheet-sterile-gloves-rodent-surgery\/","title":{"rendered":"Helpful Information Sheet: Sterile Gloves for Rodent Surgery"},"template":"","categories":[11,12,13,14,17],"tags":[21],"content-type":[4],"topic":[25],"update-type":[],"class_list":["post-3053","document","type-document","status-publish","hentry","category-guinea-pigs","category-hamsters","category-mice","category-other-rodents","category-rats","tag-u-m-resource","content-type-informational","topic-surgery"],"acf":{"use_legacy_editor":true,"updated_date":"2015-12-18 14:00:00","update_notice":false,"author":"Animal Care &amp; Use Program","summary":"The information contained within this document is intended to supplement the <a href=\"https:\/\/az.research.umich.edu\/animalcare\/guidelines\/guidelines-performance-surgery-rodents\/\"><em>Guidelines on the Performance of Surgery in Rodents<\/em><\/a>, which require \"the use of sterile, individually packaged gloves for rodent (e.g., mice, rats, hamsters, gerbils, guinea pigs, etc.) surgery.\"","button_links":null,"related_content":[2891,2984],"legacy_path":"helpful-information-sheet-sterile-gloves-rodent-surgery","legacy_node_id":1384,"legacy_related_nids":"36, 136","legacy_content_section":[{"legacy_section_type":"heading","legacy_heading":"Helpful Information to Meet the Guideline Standards","legacy_subheading":"","legacy_section_text":"","legacy_media_position":"","legacy_media_file":"","legacy_media_url":"","legacy_glossary_term":"","legacy_glossary_nids":"","legacy_resource":"","legacy_resource_nids":"","legacy_buttons":null},{"legacy_section_type":"text_area","legacy_heading":"","legacy_subheading":"","legacy_section_text":"<strong>Sterile gloves can be purchased directly from commercial suppliers.<\/strong>\r\n<ul>\r\n \t<li>Commercially available, individually packaged pairs of exam gloves are available via:\r\n<ul>\r\n \t<li>Amazon (<a href=\"http:\/\/www.amazon.com;\">www.amazon.com;<\/a> search for \u201csterile exam glove\u201d)<\/li>\r\n \t<li>eSafetySupply (<a href=\"http:\/\/www.esafetysupply.com;\">www.esafetysupply.com;<\/a> follow menu Category \u2192 Disposable Gloves \u2192 Latex Gloves \u2192 Medical\/Exam Grade Latex Gloves \u2192 Sterile Gloves<\/li>\r\n<\/ul>\r\n<\/li>\r\n \t<li>Please note that gloves specifically manufactured for surgery are <strong>not<\/strong> required, but are acceptable.<\/li>\r\n \t<li>Regular exam gloves, packaged and autoclaved with appropriate quality assurance measures to ensure sterility (e.g., autoclave tape, etc.) are also acceptable.<\/li>\r\n<\/ul>","legacy_media_position":"","legacy_media_file":"","legacy_media_url":"","legacy_glossary_term":"","legacy_glossary_nids":"","legacy_resource":"","legacy_resource_nids":"","legacy_buttons":null},{"legacy_section_type":"heading","legacy_heading":"Guidance for Sterilizing Gloves for Rodent Surgeries","legacy_subheading":"","legacy_section_text":"","legacy_media_position":"","legacy_media_file":"","legacy_media_url":"","legacy_glossary_term":"","legacy_glossary_nids":"","legacy_resource":"","legacy_resource_nids":"","legacy_buttons":null},{"legacy_section_type":"text_area","legacy_heading":"","legacy_subheading":"","legacy_section_text":"<strong>The guidance below provides information on how to appropriately sterilize examination gloves.<\/strong>\r\n<h4>Supplies Needed<\/h4>\r\n<ul>\r\n \t<li>Clean latex or nitrile exam gloves<\/li>\r\n \t<li>Paper drape material<\/li>\r\n \t<li>Sterilization pouches<\/li>\r\n \t<li>Autoclave indicator<\/li>\r\n \t<li><a class=\"gtip\" href=\"\/animalcare\/glossary\/autoclave\">Autoclave<\/a><\/li>\r\n<\/ul>\r\n<h4>Step-by-Step Instructions<\/h4>\r\n<table style=\"border-collapse: collapse;width: 100%\">\r\n<tbody>\r\n<tr>\r\n<td style=\"width: 4.80981%;vertical-align: top;text-align: center\"><strong>1.<\/strong><\/td>\r\n<td style=\"width: 95.19%;vertical-align: top\" colspan=\"2\">Assure that autoclave is in good working order, with periodic testing, assessment, and appropriate record-keeping (review the\u00a0<strong><a href=\"https:\/\/az.research.umich.edu\/animalcare\/informational\/monitoring-effectiveness-autoclaves\/\"><em>Monitoring Effectiveness of Autoclaves<\/em><\/a><\/strong>\u00a0document for more information)<\/td>\r\n<\/tr>\r\n<tr>\r\n<td style=\"width: 4.80981%;vertical-align: top;text-align: center\"><strong>2.<\/strong><\/td>\r\n<td style=\"width: 95.19%;vertical-align: top\" colspan=\"2\">Fold the wrists of each glove up over the hand of the glove<\/td>\r\n<\/tr>\r\n<tr>\r\n<td style=\"width: 4.80981%;vertical-align: top;text-align: center\"><strong>3.<\/strong><\/td>\r\n<td style=\"width: 95.19%;vertical-align: top\" colspan=\"2\">Cut drape material into rectangles to fold around each pair of gloves<\/td>\r\n<\/tr>\r\n<tr>\r\n<td style=\"width: 4.80981%;vertical-align: top;text-align: center\"><strong>4.<\/strong><\/td>\r\n<td style=\"width: 66.2192%;vertical-align: top\">Wrap gloves, one pair per packet, with drape material to separate pairs of gloves or to separate gloves from instruments<\/td>\r\n<td style=\"width: 28.9708%\"><img src=\"https:\/\/az.research.umich.edu\/animalcare\/wp-content\/uploads\/sites\/2\/2025\/01\/acu-sterilizegloves-step4.png\" alt=\"acu-sterilizegloves-step4.png (1000\u00d7750)\" \/><\/td>\r\n<\/tr>\r\n<tr>\r\n<td style=\"width: 4.80981%;vertical-align: top;text-align: center\"><strong>5.<\/strong><\/td>\r\n<td style=\"width: 66.2192%;vertical-align: top\">Place an autoclave indicator within each glove<\/td>\r\n<td style=\"width: 28.9708%\"><img src=\"https:\/\/az.research.umich.edu\/animalcare\/wp-content\/uploads\/sites\/2\/2025\/01\/acu-sterilizegloves-step5.png\" alt=\"acu-sterilizegloves-step5.png (1000\u00d7750)\" \/><\/td>\r\n<\/tr>\r\n<tr>\r\n<td style=\"width: 4.80981%;vertical-align: top;text-align: center\"><strong>6.<\/strong><\/td>\r\n<td style=\"width: 66.2192%;vertical-align: top\">Place up to 5 pairs of gloves in each sterilization pouch, or one pair with the instruments to be used for the surgery\r\n\r\n(Note: contrary to the picture, place gloves <em><strong>ON TOP OF<\/strong><\/em> instruments to assist in maintaining sterility)<\/td>\r\n<td style=\"width: 28.9708%\"><img src=\"https:\/\/az.research.umich.edu\/animalcare\/wp-content\/uploads\/sites\/2\/2025\/01\/acu-sterilizegloves-step6.png\" alt=\"acu-sterilizegloves-step6.png (1000\u00d7750)\" \/>\r\n\r\n<img src=\"https:\/\/az.research.umich.edu\/animalcare\/wp-content\/uploads\/sites\/2\/2025\/01\/acu-sterilizegloves-step6.1.png\" alt=\"acu-sterilizegloves-step6.1.png (1000\u00d7750)\" \/>\r\n\r\n<img src=\"https:\/\/az.research.umich.edu\/animalcare\/wp-content\/uploads\/sites\/2\/2025\/01\/acu-sterilizegloves-step6.2.png\" alt=\"acu-sterilizegloves-step6.2.png (1000\u00d7750)\" \/>\r\n\r\n<img src=\"https:\/\/az.research.umich.edu\/animalcare\/wp-content\/uploads\/sites\/2\/2025\/01\/acu-sterilizegloves-step7.png\" alt=\"acu-sterilizegloves-step7.png (1000\u00d7750)\" \/><\/td>\r\n<\/tr>\r\n<tr>\r\n<td style=\"width: 4.80981%;vertical-align: top;text-align: center\"><strong>7.<\/strong><\/td>\r\n<td style=\"width: 95.19%;vertical-align: top\" colspan=\"2\">Autoclave gloves\r\n<ul class=\"rteindent1\">\r\n \t<li>Vacuum cycles\r\n<ul class=\"rteindent1\">\r\n \t<li>Sterilize at 121\u00b0C for 20 minutes and dry 12 minutes<\/li>\r\n \t<li>Sterilize at 121\u00b0C for 30 minutes and dry 12 minutes<\/li>\r\n \t<li>Sterilize at 132\u00b0C for 6 minutes and dry 20 minutes<\/li>\r\n \t<li>Sterilize at 132\u00b0C for 3 minutes and dry 1 minute (flash cycle used only for gloves in a peel pouch \u2013 no instruments)<\/li>\r\n<\/ul>\r\n<\/li>\r\n \t<li><a class=\"gtip\" href=\"\/animalcare\/glossary\/gravity-or-prevac-cycle\">Gravity Cycle<\/a>\r\n<ul class=\"rteindent1\">\r\n \t<li>Sterilize 30 minutes and dry 10 minutes<\/li>\r\n<\/ul>\r\n<\/li>\r\n<\/ul>\r\n<\/td>\r\n<\/tr>\r\n<\/tbody>\r\n<\/table>\r\nFor more information about the preparation of surgical supplies for rodent surgery, including the use of surgical drapes and instruments, please refer to the <strong><a href=\"https:\/\/az.research.umich.edu\/animalcare\/guidelines\/guidelines-performance-surgery-rodents\/\"><em>Guidelines on the Performance of Surgery in Rodents<\/em><\/a>.<\/strong>","legacy_media_position":"","legacy_media_file":"","legacy_media_url":"","legacy_glossary_term":"","legacy_glossary_nids":"","legacy_resource":"","legacy_resource_nids":"","legacy_buttons":null}],"update_notice_type":[],"update_notice_start":"","update_notice_end":"","update_notice_text_blocks":null,"global_contact_block":false,"contact_name":"","contact_email":"","contact_additional_info":"If you have questions or comments about this document, or about the use of sterile gloves in rodent surgery, contact\u00a0<strong>ULAM Veterinary Staff<\/strong> at <a href=\"mailto:ulam-vets@umich.edu\">ulam-vets@umich.edu<\/a>.","global_contact_block_select":null},"_links":{"self":[{"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/document\/3053","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/document"}],"about":[{"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/types\/document"}],"version-history":[{"count":1,"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/document\/3053\/revisions"}],"predecessor-version":[{"id":4009,"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/document\/3053\/revisions\/4009"}],"acf:post":[{"embeddable":true,"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/document\/2984"},{"embeddable":true,"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/document\/2891"}],"wp:attachment":[{"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/media?parent=3053"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/categories?post=3053"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/tags?post=3053"},{"taxonomy":"content-type","embeddable":true,"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/content-type?post=3053"},{"taxonomy":"topic","embeddable":true,"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/topic?post=3053"},{"taxonomy":"update-type","embeddable":true,"href":"https:\/\/az.research.umich.edu\/animalcare\/wp-json\/wp\/v2\/update-type?post=3053"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}