To establish acceptable methods of euthanasia for investigative personnel to perform. The current procedure is composed of two parts; a primary method followed by a secondary method to ensure death and/or prevent revival.
This document is designed to provide general guidelines about administration of substances to laboratory animals. All procedures must be approved by the Institutional Animal Care & Use Committee (IACUC). The route of administration, intervals between substance administration, dose range, and volume to be administered should be listed in the approved protocol specific to each study.
This document is designed to provide general information on blood collection methods for common laboratory animals. All procedures must be approved by the Institutional Animal Care and Use Committee (IACUC). The method of blood collection to be used, the intervals between blood collection procedures, and the volume of blood to be removed, must be listed in the approved protocol specific to each study.
The ULAM veterinary staff provides the following criteria to determine the maximum safe amount of blood to be withdrawn. It is recommended to take no more blood than is absolutely necessary. Remember to calculate beforehand the minimum amount of blood necessary to perform all tests and assays, and that the serum fraction is about ½ of the total blood volume. When calculating blood volumes based on body weights, remember that body weights in kilograms (kgs) will convert to blood volumes in liters, and weights in grams will convert to volumes in milliliters (mls).
To establish a standard procedure for euthanasia of a laboratory animal by ULAM veterinary staff when it is determined that euthanasia is the most humane treatment. Information is also provided on the appropriate means to communicate with laboratories regarding specific animal cases.
The intent of this policy is to identity the requirements regarding conscious, unanesthetized physical restraint of animals used in research, teaching or testing activities at the University of Michigan.
These guidelines are based on the AVMA Guidelines for the Euthanasia of Animals (2013 Edition). Abbreviation codes are as follows:
A = Acceptable - those which consistently produce a humane death when used as the sole means of euthanasia
AC = Acceptable with Conditions - those which consistently produce a humane death when specific conditions are met
ACJ = Acceptable with Conditions requiring Justification - may not consistently produce a humane death as sole means and requires IACUC approval
U = Unacceptable - deemed inhumane under any conditions, or found to pose substantial risk to human applying the technique