Entry and Exiting Animal Housing Areas
Procedures for entry and exiting animal housing areas, including changing the Vivarium Dedicated Uniform and showering.
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Responsibilities
- Husbandry Personnel
- Veterinary Personnel
- Investigative Personnel
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Glossary Definitions
Conventional Animals
Animals with an undetermined microbiological status; animals are raised and maintained under standard conditions.
Disinfectant
Bleach solution or Clidox©.
Donning / Doffing
The practice of personnel putting on and removing work-related protective gear, clothing and uniforms.
Germ-Free Animals
Animals that have no microorganisms living in or on them and are raised within germ-free isolators in order to control their exposure to viral, bacterial, or parasitic agents.
Green or Clean Rooms
Animal rooms clean from any contaminants such as MPV, Pinworm, Orf, Q-Fever etc.
Micro-isolation (MI) Technique
A method of housing and handling animals to maintain them free of disease-causing pathogens.
Non-Rodent Mammal
Rabbit, ferret, dog, cat, swine, sheep, cow, non-human primate, etc.
Non USDA-Regulated Species
A term that refers to animals whose care is NOT governed by the Animal Welfare Act. This includes rats of the genus Rattus and mice of the genus Mus bred for research, birds, fish, reptiles, and amphibians.
Nude/SCID rooms
Room that houses immunodeficient mice.
Quarantine Animal
Animals with disease or unknown health status.
Red or Contaminated Rooms
Animals in rooms known to be contaminated with a virus, parasite or bacteria. Red sign indicates the contamination status and is placed on the door.
Specific Pathogen Free (SPF)
Animals which are free of the specific pathogens tested for in the Unit for Laboratory Animal Medicine's Rodent Health Surveillance Program.
Yellow or Potentially Contaminated Rooms
Previously contaminated animal room and is now believed to be free from any contaminants. Prior to contamination status release, surveillance results must confirm "clean" status. Certain viral outbreaks may have insufficient information regarding the virus and require animal rooms to placed in "potentially contaminated" state at the discretion of the area veterinarian.
Vivarium Dedicated Uniform (VDU)
Long sleeve t-shirts, VDU, or other clothing that is designated as personal protective equipment to be worn and laundered within the animal vivarium space.
USDA-Regulated Species
A term that refers to animals whose care is governed by the Animal Welfare Act. USDA-regulated Species include all live or dead warm-blooded animals used in research except birds, rats of the genus Rattus, and mice of the genus Mus bred for research. This also excludes "cold-blooded" animals such as fish, reptiles, and amphibians.
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Procedures
1. Animal and Euthanasia Room Entry Procedures
- Ensure all entry room procedures are followed to maintain the health status of the animals.
- Hang personal items such as lab coats, jackets, bags and hats outside the room.
- Follow the designated personal protective equipment (PPE) instructions listed on the room entry procedures sign posted on the outside of the door and/or area entrance.
- Ensure proper attire is worn (long pants/skirt or equivalent leg covering and closed toed shoes).
- Euthanasia rooms require additional PPE over VDU.
- Don gown before gloves. Utilize head and thumb openings, if present.
- Pull gloves over the cuff of the gown.
- Discard gown if falls on the floor.
- Follow building specific procedures for location of donning and doffing PPE.
- PPE requirements may change during an outbreak.
- Change PPE between species.
- Area faculty veterinarians approve any deviations that may need to occur.
- Post approved deviations on a Special Treatment or Procedure form (SToP).
- Area faculty veterinarians approve any deviations that may need to occur.
- Consult the area faculty veterinarian if there is a change in the health status of a room.
- Leave laboratory carts outside of the housing areas of:
- C. bovis free rooms
- Nude/SCID rooms
- Biocontainment rooms
2. Animal Room Entry Order
- Rodent Room Entry Order:
- Enter rooms, within the same workday, as follows:
- Germ-Free
- C. bovis free
- Nude or SCID
- SPF clean room (Green)
- SPF potentially contaminated (Yellow)
- The following rooms are of equal health status and can be entered in any order between SPF potentially contaminated (Yellow) and conventional rooms:
- SPF biocontainment
- SPF contaminated (Red)
- Quarantine
- Conventional
- Exceptions to the room entry order:
- Enter prairie vole rooms AFTER all other rodent and rabbit rooms.
- Wash hands up to elbows and change VDU if there is a need to return to another rodent or rabbit room after entering a prairie vole room.
- Enter prairie vole rooms AFTER all other rodent and rabbit rooms.
- PPE in Rodent Rooms – Non-USDA Regulated Species
- With a LFH and microisolator caging:
- VDUs and gloves, or
- Designated personal protective equipment (PPE) as listed on the room entry procedures sign
- Without a LFH and/or microisolator caging AND when cages are being opened:
- Designated personal protective equipment (PPE) as listed on the room entry procedures sign
- VDUs and gloves are acceptable when cages are not being opened.
- With a LFH and microisolator caging:
- PPE in Rodent Rooms – USDA Regulated Species
- Designated personal protective equipment (PPE) as listed on the room entry procedures sign
- Enter rooms, within the same workday, as follows:
- Non-rodent Mammal Room Entry Order:
- PPE in Rabbit Rooms
- Wear disposable CLOTH gowns.
- Change gowns when torn and/or soiled.
- Wear disposable CLOTH gowns.
- Enter rabbit rooms AFTER guinea pig rooms.
- All personnel change VDU if there is a need to return to a guinea pig room after entering a rabbit room.
- Enter non-rodent mammal rooms (per species) in quarantine LAST within a workday.
- Ensure PPE is changed between all non-rodent mammal rooms.
- PPE in Rabbit Rooms
- Alternate PPE is provided by the individual’s department if personnel are unable to wear the PPE as required and if deemed to be at least as effective as the required equipment. Personnel are not able to conduct the work tasks in which PPE is deemed necessary if unable to wear required PPE or alternate PPE is not acceptable. Consult EHS, the faculty veterinarian, and the employee.
- Animal Room Exiting Procedures:
- Remove and dispose of all PPE immediately prior to exiting the room, pod, hall, or other designated area.
3. Animal Room Contamination
- Rodent Contamination:
- Place appropriate contamination signage on the door of the animal room if contaminants are present.
- Include don/doff procedures on door signage.
- Don a disposable gown and gloves PRIOR to entering a room with confirmed contaminations.
- Doff PPE based on room signage.
- Place appropriate contamination signage on the door of the animal room if contaminants are present.
- Non-rodent Mammal Contaminations:
- Place appropriate contamination signage on the door of the animal room if contaminants are present.
- Include don/doff procedures on door signage.
- Don required PPE PRIOR to entering a room with confirmed contaminations.
- Additional PPE must be worn over VDU.
- Place appropriate contamination signage on the door of the animal room if contaminants are present.
4. Personal Hygiene and Clothing Procedures for ULAM Personnel
- Showering:
- Shower, including washing hair, and don clean VDU prior to entering a Nude/SCID rodent room after working in an SPF contaminated (Red) rodent room or conventional animal room.
- Clothing
- General VDU Procedures
- Discard VDU in dirty laundry bin at the end of the workday, or at any other required time that VDUs must be changed, to be laundered per building procedures.
- Wash and dry VDU in research areas only. VDU must not be removed from the University of Michigan campus.
- Change VDU when traveling backward in room entry order, e.g. travelling from SPF clean room (Green) to Nude/SCID room (See Procedures section 2.a.i.1 thru 7).
- Disinfect shoes/boots with an appropriate disinfectant when entering an SPF large animal room after a quarantine or biocontainment large animal housing room.
- Change VDU PRIOR to breaks and lunches if travelling from:
- Contaminated rooms
- Conventional rooms
- Dirty side cage wash
- Change VDU PRIOR to leaving the vivarium to enter a public space unless:
- Traveling from one animal area to another.
- Additional PPE is worn over VDU while working within the animal facility.
- Change soiled or wet VDU after completing current task or as soon as possible.
- Change VDU AFTER break, lunch, or meeting if duties involve working in clean side cage wash.
- Change VDU when working in between different dirty side cage wash areas.
- General VDU Procedures
- Ensure all entry room procedures are followed to maintain the health status of the animals.
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Appendix A: Animal Room Status Color Indicator Descriptions
Red: Contaminated
- Room pressure: refer to the CODA worksheet FINAL in the ULAM Cafe
- The room is contaminated, or the contamination has been removed, but the room has not yet been disinfected. Depending upon the contamination, suspect animals are tested before being moved out of a RED room or shipped.
- Animals testing negative can be moved to a Yellow or Green room. Animals testing positive should be culled, rederived, or treated.
- New Arrivals may be redirected to a Green room for the duration of the contamination.
Yellow: Potentially Contaminated
- Room pressure: refer to the CODA worksheet FINAL in the ULAM Cafe
- Rooms with the contamination removed that have been disinfected but do not have new surveillance results back. Rooms that received arrivals from contaminated rooms before we learned about the contamination.
- Room remains at Yellow status until the next round of surveillance comes back negative. Animals can be moved to other Yellow rooms, or to Red rooms.
- Animals testing negative can be moved to Green rooms.
Green: Clean
- Standard housing rooms without contamination.
- Rooms with the contamination removed that have been disinfected and have negative post-cleanup surveillance results.
- Animals can be moved from any Green room to Green, Yellow, or Red rooms. If they are moved to a Yellow or Red room, they cannot move back to a Green room.
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Appendix B: Animal Room Contamination Sign
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Appendix C: Animal Room Potential Contamination Sign
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Appendix D: PPE Risk Prioritization
Questions?
Questions or concerns about the content of this document should be directed to the Unit for Laboratory Animal Medicine (ULAM) at (734) 764-0277 or ulam-questions@umich.edu.
