SOPs

SOP for Laboratory Assumption of the Feeding, Watering and/or Cage Changing of Rodents

Unit for Laboratory Animal Medicine
Feb 10, 2020 12:00 am

The Unit for Laboratory Animal Medicine (ULAM) has developed a policy by which the staff of a research laboratory can feed and/or water the rodents used in their research project(s), and thus not use ULAM husbandry for these services.

  • Responsibility

    1. ULAM Husbandry Personnel
    2. ULAM Investigative Personnel
  • Glossary Definitions

    Lab Will Care Log

    This form serves as the log sheet for research personnel to indicate care (feed, water, cage change) provided to the animals. These are typically placed on the inside of the animal room door.

    Special Treatment or Procedure (SToP) Form

    A form that indicates special procedure differing from standard operating procedure (SOP). These are typically placed on the inside of the animal room door. See Request for Special Treatment or Procedure (SToP) Form for more information.

    Task Codes

    Codes used by lab personnel to indicate the type of care provided.

    • F: Feed provided (restricted or topped off)
    • W: Water bottle topped off
    • CW: Changed water bottle
    • CC: Cage change performed
    • S: New cage started
  • Procedures

    1. Principal Investigator must sign a copy of the Memorandum of Understanding (MoU) under Appendix A, acknowledging that they understand the rules and stipulations related to providing care (feed, water, and caging) to rodents.
    2. Investigative Personnel must complete a SToP Form, which details the changes in feeding and/or watering practice and the rationale supporting those changes, and a Lab Will Care Log, used to document the care provided.
      1. Principal Investigator or Investigative personnel provides a list of contact information (preferred contact number and email addresses) for all relevant lab staff.
      2. Document on the Lab Will Care Log any special water or diet requirements (and in some cases, what types of food and water cannot be provided).
      3. Location of the water or diet supply is in case of an emergency.
    3. Investigative personnel must identify each cage they intend to provide care for using a Lab Will Care label.
      1. Must indicate on label which aspect of care investigative personnel will be responsible (i.e. Feed, Water, Cage Change) and if feed/water will be restricted or ad lib.
        1. Administer food and/or water to animals per SToP form specifications (i.e., restricted or non-restricted diets)
        2. If food and/or water is restricted, investigative personnel must check animals daily for presence of food and/or water.
        3. Administering food and/or water that is not restricted, documentation occurs when food and/or water is given (may be every few days).
        4. Cage changing must be performed as outlined in Rodent Equipment Maintenance and Sanitization SOP.
      2. Must also indicate a Cage Identification number (Cage ID #) on label
    4. Investigative personnel must document on the Lab Will Care Log when care is provided
      1. For each entry, complete the following:
        1. Date
        2. Cage ID #
        3. Task code
        4. Initials of the person performing the task.
      2. Multiple cage ID’s can be listed in a single entry if the same care is provided to each cage
        1. i.e. Cages 1-5 all received a cage change: code as “1-5: CC”
    5. ULAM husbandry personnel evaluate rodents as documented in the Rodent Husbandry SOP.
      1. If it is clear tasks have been completed as described but no documentation has been noted on the Lab Will Care Log, ULAM husbandry personnel contacts laboratory staff requesting documentation reflect tasks completed
    6. If ULAM husbandry personnel find rodents without food or water or are low on food or water and are being cared for by the laboratory staff, as based on a SToP Form, do the following:
      1. Check the Lab Will Care label for indication of a restriction to food or water.
      2. If documentation on the Lab Will Care Log form occurred within the past 24 hours, ULAM husbandry personnel do not need to contact the lab.
      3. If documentation has not occurred within the past 24 hours proceed with the steps below:
        1. ULAM husbandry personnel contacts investigative personnel
          1. Include rack number and slot number of cage(s) needing attention.
        2. If investigative personnel CAN be reached and the cage has not been fed/watered by the following day, ULAM husbandry personnel provides food/water.
          1. Locate, identify and provide special feed/water (noted on SToP form) to the animals. If the lab’s feed/water cannot be located or identified, provide commercial rodent chow (Lab Diet #5LOD)/water as routinely provided to University animals.
          2. Label the cage(s) with a yellow acetate placed in the vertical (“up”) position in the cage card holder. The acetate will be labeled “ULAM watered (or fed) at [date/time]” This acetate will remain on the cage for 72 hours.
          3. ULAM husbandry personnel must also make a note for the affected Cage ID’s on the Lab Will Care Log indicating “ULAM (watered or fed) at [time] using [note type of water or food]"
          4. Charge technician time per the Technical Time Charge Report SOP
        3. If investigative personnel CANNOT be contacted by 2:00 PM on Monday thru Friday or 10:30 AM on Saturday, Sunday, and holidays, follow these procedures:
          1. Rodents needing food/water: Provide food/water before the end of the workday (2:30 PM Monday thru Friday; or as close to end time as possible (e.g.11:00 am) on Saturday, Sunday, and holidays)
          2. Follow steps 6.c.ii.1 through 6.c.ii.4 for providing food/water, proper documentation and tech time charges
    7. If ULAM husbandry personnel find cages wet and are being cared for by investigative personnel, as based on a Lab Will Care SToP form, do the following:
      1. If cage is ¼ wet or more, but there is no standing water, proceed with the steps below:
        1. ULAM husbandry personnel contact investigative personnel.
          1. Include rack number and slot number of cage(s) needing changed.
          2. If investigative personnel CAN be reached and the cage has not been changed by the following day, ULAM husbandry personnel will perform cage change.
            1. Label the cage(s) with a yellow acetate placed in the vertical (“up”) position in the cage card holder. The acetate will be labeled “ULAM cage change at [date/time]” This acetate will remain on the cage for 72 hours
            2. ULAM husbandry personnel must also make a note for the affected Cage ID’s on the Lab Will Care Log indicating “ULAM CC” at [time].
            3. Charge technician time per the Technical Time Charge Report SOP
        2. If investigative personnel CANNOT be contacted by 2:00 PM on Monday thru Friday or 10:30 AM on Saturday, Sunday, and holidays, follow these procedures:
          1. ULAM staff changes the cage
            1. Follow steps 7.a.i.2.a through 7.a.i.2.c for performing cage changes, proper documentation and tech time charges
      2. If cage has standing water, ULAM husbandry personnel performs cage change immediately.
        1. Follow steps for handling flooded cages as outlined in Rodent Husbandry SOP.
        2. Follow steps 7.a.i.2.a through 7.a.i.2.c for performing cage changes, proper documentation and tech time charges.
    8. If ULAM husbandry personnel must separate a Lab Will Care cage (overcrowded conditions, fighting, weaning, etc.) follow these procedures:
      1. Ensure the new cage conditions (bedding, food, water, enrichment, etc.) are identical to original cage.
        1. If food or water is restricted follow procedures outlined above.
      2. Label the cage(s) with a yellow Lab Will Care acetate, copying the information from original cage acetate, minus the Cage ID.
      3. Send an email to the lab contacts listed on the associated SToP form.
        1. Include in the email:
          1. The location of the new cage
          2. Whether the new cage needs special food or water added
          3. The need for a new Cage ID
  • Appendix A: Memorandum of Understanding - Lab Assumes Care

  • Appendix B: Lab Will Care Log

Species: Mice Rats
Questions?

Questions or concerns about the content of this document should be directed to the Unit for Laboratory Animal Medicine (ULAM) at (734) 764-0277 or ulam-questions@umich.edu.